Research Associate/Senior Research Associate, Genome Engineering

An affiliate of Colossal is seeking an experienced Research Associate/Senior Research Associate to support precision genome engineering in non-model mammalian organisms. You will produce and validate CRISPR/Cas9, prime editing, base editing, and multiplex editing reagents that enable Cell Engineering colleagues to generate edited cell lines. We seek a technically proficient, hands-on contributor who can execute established workflows independently and contribute from the outset.

This position is based on-site in Dallas, TX. Relocation assistance is available.

Duties and Responsibilities:

• Generate and quality-control genome editing reagents, including sgRNAs, pegRNAs, donor DNA templates, plasmids, and expression constructs
• Perform molecular cloning, DNA assembly, bacterial culture, plasmid purification, PCR, and sequence verification
• Support preparation and quality control of RNA reagents, including in vitro transcription and purification where applicable
• Prepare and track reagent sets for multiplex genome editing experiments, maintaining accurate construct and sample identities
• Work with Genome Engineering scientists to implement and troubleshoot CRISPR/Cas9, prime editing, and base editing designs
• Transfer validated reagents and clear experimental documentation to Cell Engineering colleagues for transfection and downstream cellular workflows
• Perform PCR-based genotyping, prepare samples for sequencing, and support analysis of single-locus and multiplex editing outcomes
• Maintain accurate electronic laboratory records, reagent inventories, and quality-control documentation in ELN
• Organize concurrent experiments, communicate results, and proactively improve workflows

Required Education, Skills, and Abilities:

• BA/BS or MA/MS in molecular biology, genetics, biotechnology, bioengineering, or a related field
• Demonstrated hands-on molecular biology research experience, including cloning, PCR, plasmid preparation, and sequence verification
• Practical experience supporting CRISPR/Cas9 genome editing and multiplex editing experiments or reagent workflows
• Experience preparing, handling, and quality-controlling DNA and/or RNA editing reagents
• Ability to independently execute established protocols, troubleshoot routine technical problems, and contribute immediately with limited training
• Strong organizational skills, attention to detail, and written and oral communication skills

Preferred Skills and Abilities:

• Hands-on experience with prime editing and/or base editing reagents and workflows
• Experience designing or preparing pegRNAs, modified guide RNAs, or HDR donors
• Experience with in vitro transcription, RNA purification, or nucleic acid QC
• Experience with sequencing-based genotyping and analysis of multiplex edits
• Experience with electronic laboratory notebooks and cross-functional reagent handoffs