Scientist I, Guide RNA Chemistry

Company Overview: Prime Medicine is a leading biotechnology company dedicated to creating and delivering the next generation of gene editing therapies to patients. The Company is leveraging its proprietary Prime Editing platform, a versatile, precise and efficient gene editing technology, to develop a new class of differentiated, one-time, potentially curative genetic therapies. Designed to make only the right edit at the right position within a gene while minimizing unwanted DNA modifications, Prime Editors have the potential to repair almost all types of genetic mutations and work in many different tissues, organs and cell types.

Prime Medicine is currently progressing a diversified portfolio of investigational therapeutic programs organized around our core areas of focus: hematology, immunology & oncology, liver and lung. Across each core area, Prime Medicine is focused initially on a set of high value programs, each targeting a disease with well-understood biology and a clearly defined clinical development and regulatory path, and each expected to provide the foundation for expansion into additional opportunities. com .

Prime Medicine was founded to bring the promise of gene editing to patients. We use Prime Editing, a next-generation technology that can “search and replace” to restore normal genetic function almost anywhere in the genome. Prime Editing searches to find the precise place in the genome to edit and replaces the segment of faulty DNA with a correct copy of DNA.

A single Prime Editor can correct the individual mutations found across patients, meaning Prime Editing can potentially address more than 90 percent of known disease-causing genetic mutations. We envision a world where this technology can cure, halt, and ultimately prevent genetic diseases, providing lifelong benefit to patients. Prime Medicine offers the opportunity to shape the future of gene editing and transform how medicines are used to treat disease.

Realizing the promise of Prime Editing requires a talented team with diversity of viewpoints and expertise. We’re building a group of dedicated, scientifically curious individuals who are passionate about helping patients live longer, healthier lives. Position Overview Prime Medicine is seeking a talented, highly motivated Scientist I with expertise in oligonucleotide chemistry to join our Guide Chemistry team.

The successful candidate will have hands-on experience in long guide RNA synthesis, chemical deprotection, purification, desalting, and the implementation of novel chemistries to enhance guide RNA potency and quality, along with proficiency operating MerMade 12 and/or Dr. Oligo synthesizers for solid-phase RNA synthesis using phosphoramidite chemistry, followed by HPLC purification to support multiple discovery programs. This role requires a strong understanding of phosphoramidite chemistry, solid-phase oligonucleotide synthesis, linker chemistry, enzymatic ligation, and RNA purification techniques to enable guide RNA production for in vivo studies, preclinical development, and beyond.

The Scientist I will play a key role in troubleshooting technical challenges, improving throughput and process robustness, and ensuring reliable guide-RNA production for screening and animal studies. The individual will leverage scientific literature, collaborate with internal technical experts, and independently solve complex laboratory problems. Depending on experience, this individual may mentor co-ops, contractors, or junior scientists and will work closely with cross-functional teams including Analytical Development, Biology, Formulation, and Technical Development & Operations (TDO) to advance Prime Medicine's gene editing programs toward the clinic.

Key Responsibilities

Design, plan, and execute complex laboratory experiments that support cross-functional research objectives.