Scientist II, Cell Line Engineering

The role: BigHat is looking for an energetic and motivated individual to lead cell line engineering as a core Platform capability in support of our pipeline Discovery and early R&D efforts. This is a lab based role that will report directly to the Head of Protein Sciences, work cross functionally with Programs and Discovery, and directly impact the identification and validation of next generation biotherapeutics engineered on the Milliner Platform.

Responsibilities

and title would adjust as appropriate. Onboard all the core components necessary for engineering cell lines for expressing pipeline targets including expression vectors, gene delivery methods, selective antibiotic concentration determination protocols, expression validation and cell sorting capabilities Culture and maintain a variety of cell types; generate diverse target cell lines including knock-outs, stable cell lines overexpressing one or more targets, and reporter cell lines Perform the validation of cell line expression using flow and sequencing (Sanger or NGS) based methods. Generate bulk pool and single cell populations utilizing FACS to select for cell lines of various expression levels Maintain an organized cell line inventory with all relevant documentation Investigate target expression biology to recapitulate the most native conditions for expression.

Work with end user stakeholders to confirm correct domain expression and associated sequences In collaboration with end user stakeholders, establish the workflows, timelines and deliverables for cell line engineering from request to hand-off Collaborate with automation engineers to automate routine cell culture workflows to increase efficiencies and throughput PhD in biology-related discipline with minimum of 3+ years industry experience, preferably in an environment utilizing cell lines for antibody discovery and engineering In depth molecular biology knowledge for plasmid construction and experience selecting expression vectors and promoter types Experience culturing various cell types, including adherent, suspension, shaker flask etc.

Benefits

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through Nayya, OneMedical, Wagmo, Rula, and more 401(k) with company match DTO, two weeks of company-wide shutdown, and 12 company holidays Paid parental leave

Compensation

will vary depending on job-related knowledge, skills, and experience.

will be confirmed in writing at the time of the offer.